The Lineweaver-Burk plot is a graphical illustration of the Michaelis-Menten equation, which describes the connection between the response charge of an enzyme-catalyzed response and the substrate focus. The alpha worth in a Lineweaver-Burk plot is the x-intercept and represents the destructive inverse of the Michaelis fixed (Okaym). The Okaym worth is a measure of the affinity of the enzyme for its substrate, and a decrease Okaym worth signifies the next affinity. Subsequently, the next alpha worth signifies a decrease Okaym worth and the next affinity of the enzyme for its substrate.
The Lineweaver-Burk plot is a great tool for figuring out the kinetic parameters of an enzyme-catalyzed response. It may be used to find out the Vmax, the utmost response charge, and the Okaym, the Michaelis fixed. The Vmax is the utmost velocity of the response, and it’s reached when the enzyme is saturated with substrate. The Okaym is the substrate focus at which the response charge is half of the Vmax.
